Praja1, SUMO-tag Recombinant
Catalog #
81055
$465
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Description
Human Praja1 recombinant protein (Genbank Accession No. NP_660095), full length with N-terminal SUMO-tag, expressed in E. coli.
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Synonyms
RNF70, RING finger protein 70
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
E. coli
Purity
≥85% by SDS-PAGE
Format
Aqueous buffer solution
Formulation
50 mM Tris Buffer, pH 7.6, 150 mM NaCl, 10% glycerol
MW
71 kDa
Amino Acids
full length
Biological Activity
Typical enzyme concentration of 100 nM - 5 mM is used for in vitro conjugation, depending on conditions.
Genbank #
NP_660095
UniProt #
Q8NG27
Background
Praja1 (along with Praja2, 53% identity) is a RING-H2 domain E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E2 enzymes. Substrates for Praja1 include Dlxin-1 (MAGE-D1, human) which regulates Dlx5 dependent transcriptional function (involved in osteoblast differentiation) and ELF and Smad3 involved in the TGF-beta signaling pathway implicated in gastrointestinal cancers.
References
1. Yu, P., et al. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. Genomics, 2002. 79(6): p. 869-74.
2. Mishra, L., et al. The role of PRAJA and ELF in TGF-beta signaling and gastric cancer. Cancer Biol Ther, 2005. 4(7): p. 694-9.
3. Saha, T., et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene, 2006. 25(5): p. 693-705.
4. Sasaki, A., et al., A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. J Biol. Chem, 2002. 277(25): p. 22541-6.
2. Mishra, L., et al. The role of PRAJA and ELF in TGF-beta signaling and gastric cancer. Cancer Biol Ther, 2005. 4(7): p. 694-9.
3. Saha, T., et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene, 2006. 25(5): p. 693-705.
4. Sasaki, A., et al., A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. J Biol. Chem, 2002. 277(25): p. 22541-6.
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