PPID (CYP-40), GST-Tag Recombinant

Catalog #

71095

$370 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human Peptidyl-prolyl cis-trans isomerase D (PPID), also known as peptidyl-prolyl cis-trans isomerase Cyclophilin-40 (CYP-40, CyP-D), corresponding to amino acids 2-370(end). The construct contains an N-terminal GST-tag. The recombinant protein was affinity purified.

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Synonyms

Peptidyl-prolyl cis-trans isomerase D, PPID, peptidyl-prolyl cis-trans isomerase, Cyclophilin-40, CYP-40, CyP-D, Rotamase D

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

HSP90α: PPID Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

PPID (GST -2-370(end))

Host Species/Expression System

E. coli

Purity

≥77%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, variable glutathione, and 20% glycerol.

68 kDa

Amino Acids

2–370(end)

Genbank #

NM_005038

UniProt #

Q08752

Tag(s)

N-terminal GST-tag

Background

PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. Proposed to act as a co-chaperone in HSP90 complexes. Promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large-cell lymphoma (ALK+ ALCL) cell lines. May be involved in hepatitis C virus (HCV) replication and release.

References

1. Tavecchio, M., et al., J. Biol. Chem. 2013;288(8):5553-5561.
2. Ward, B.K., et al., J. Biol. Chem. 2002;277(43):40799-40809.

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay was performed according to HSP90α C-Terminal Domain TR-FRET Assay Kit (BPS Bioscience #50289) with HSP90α titrated from 48 ng/rxn – 0 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Metabolic Enzymes-Transferase

Regulatory Status

Research Use Only

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Datasheets/Protocols

71095 Lot 200320

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PPID (CYP-40), GST-Tag Recombinant

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