PP1A, Active, GST-tag

Catalog #: 41106
Size: 10 µg
$370
Species: Human
Host Species: Sf9 Insect Cells
MW: 62 kDa
Genbank #: NM_002708
Tag(s): N-terminal GST tag
a.a: full-length
Description:
Recombinant human serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PP1A), also known as PPP1CA and PPP1A, GenBank Accession No. NM_002708, full-length with an N-terminal GST tag and expressed in a baculovirus-infected Sf9 insect cell expression system. MW = 62 kDa.
UniProt
P62136
Synonym(s):
serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PPP1CA, PPP1A
Specific Activity:
228 pmol/min/µg
Assay Conditions:
Assay was done using BIOMOL GREEN Reagent. Briefly, 5 μl of varying amounts of PP1A was mixed with 10 μl of buffer containing 0.2% 2- mercaptoethanol and 65 ng/μl BSA and 5 μl of 1 μg/μl Thr-phosphopeptide synthetic substrate (KRT(p)IRR). Reaction was incubated at 37°C for 15 min. before adding 100 μl of BIOMOLE Green Reagent and incubating at room temp. for 30 min. Absorbance was read at 650 nm.
Formulation:
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol
Format:
Aqueous buffer solution.
Storage / Stability:
At least 6 months at –80°C.
Application(s):
Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Cohen, P. et al., J. Biol. Chem. 1989; 264 21435-21438.
2. Genoux, D. et al., Nature. 2002; 418: 970- 975.
Warning(s):
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Scientific Category:
Protein phosphatase - Serine
Product Type:
Recombinant Protein
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Product Datasheets

41106_Lot141211.pdf