Prolyl Oligopeptidase/Prolyl Endopeptidase, His-tag Recombinant

Catalog #

80105

$405 *

Size: 20 µg

Qty

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Description

Human Prolyl oligopeptidase (POP)/Prolyl endopeptidase (PREP) (GenBank Accession No. NM_002726) full length with N-terminal His-tag, MW = 81.6 kDa, expressed in an E. coli expression system.

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Synonyms

Prolyl Oligopeptidase, POP, PREP, Prolyl Endopeptidase

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

POP inhibition curve using a fluorogenic assay (#79995-1). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCL, pH 8.0, 124 mM NaCL, 2.4 mM KCL, 3 mM DTT, 10% Glycerol and variable amounts of Imidazole.

81.6 kDa

Amino Acids

full length

Specific Activity

≥ 30 pmol/min/µg.

Genbank #

NM_002726

UniProt #

P48147

Tag(s)

N-terminal His-tag

Background

Prolyl oligopeptidase (POP), also known as prolyl endopeptidase (PREP), is a cytosolic serine peptidase involved in the maturation and degradation of peptide hormones and neuropeptides.

References

1. Myohanen T.T. et al., Neurochem. Res. 32 (8), 1365-1374 (2007).
2. Garcia-Horsman J.A. et al., Scand. J. Gastroenterol. 42 (5), 562-571 (2007).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween 20, 20 µM DPP substrate 1 (A-P-AMC), 30 min at room temperature.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Protease

Regulatory Status

Research Use Only