PARP(s)

 

Poly (ADP-ribose) polymerase (PARP) is a family of proteins that, generally speaking, transfer ADP-ribose units from NAD+ onto target nuclear proteins, forming long branched Poly ADP-ribose (PAR) chains. PARPs play key signaling roles in apoptosis, DNA excision and repair, and chromatin structure. PARPs are also involved in transcriptional regulation of several signaling pathways, including genes involved in inflammation.

Some of the PARP proteins are known as tankyrases (PARP5A and 5B). In particular, tankyrases (TNKS) play a role in the maintenance of telomere length, sister telomere association, and mitotic spindle organization. Tankyrase 1 also destabilizes axin, thereby activating Wnt signaling and driving tumor cell proliferation. Thus, the PARPs, and particularly the TNKS have become important cancer drug targets. Further evidence for this comes from the fact that PARP is overexpressed in lung, ovarian, and breast cancer, and PARP inhibitors are showing dramatic therapeutic potential for breast and ovarian cancer in phase I and II clinical trials.

In addition to the role of TNKS in cancer, PARPs participate in a number of other normal and disease-associated biological processes. PARP1 binds DNMT1 and indirectly regulates DNA methylation. PARP also remodels chromatin by PARylating histones and relaxing chromatin structure. PARP2 is a substrate for histone acetyltransferases PCAF and GCN5L. PARP7 plays a role in adaptive responses to chemical exposure and mediates certain effects of the chemicals. Mice deficient in the PARP DNA repair enzyme are refractory to toxin-induced death of pancreatic beta cells and do not become diabetic. Finally, cleavage of PARP1 by caspases 3 and 7 is considered one of the classic markers of apoptosis. PARP directly induces caspase-independent apoptosis, via the production of PAR, which stimulates mitochondria to release AIF.

BPS is the only company to offer an extensive portfolio of PARP isozymes. Additionally, we offer assay kits, inhibitors, and recombinant histone substrates. Screening and profiling services are also available for over 10 different PARPs.

References:

1.    Ame, J.C., et al., BioEssays.2004; 26(8): 882-893.
2.    Rouleau, M., et al., Nat. Rev. Canc. 2010; 10: 293-301.

 

Items 26 to 50 of 87 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Catalog#SizeProduct NamePrice
52023 1 mg Histone H3, Full Length, His-tag
$465
52027 100 µg Histone H3, Full Length, His-tag
$75
52024 1 mg Histone H4, Full Length, His-tag
$465
52028 100 µg Histone H4, Full Length, His-tag
$75
52029 1 ml Histone Mixture (5X), His-tag
$310
27631-2 10 mg INO-1001
$325
27631-3 50 mg INO-1001
$710
27631-1 5 mg INO-1001
$260
27633-1 10 mg JW 55
$185
27633-2 50 mg JW 55
$380
27634-2 50 mg ME0328
$410
27634-1 10 mg ME0328
$140
80610 500 µl NAD+, Biotin-Labeled
$330
27312 50 mg NU 7026
$505
80551 96 reactions PARP1 Chemiluminescent Assay Kit
$525
80569 384 reactions PARP1 Chemiluminescent Assay Kit (384-well)
$1,080
80580 96 reactions PARP1 Colorimetric Assay Kit
$525
80521 20 μg PARP1, FLAG-Avi-tag
$425
80501 20 µg PARP1, GST-tag
$425
80560 96 reactions PARP10 Chemiluminescent Assay Kit
$1,075
80522 10 μg PARP10, FLAG-Strep-Tag
$490
80510 10 µg PARP10, His-GST-tags
$490
80561 96 reactions PARP11 Chemiluminescent Assay Kit
$1,075
80511 10 µg PARP11, GST-tag
$490
80513 10 µg PARP12, GST-tag
$490

Items 26 to 50 of 87 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4