PARP15 Chemiluminescent Assay Kit

Catalog #
80567
$1,160 *
Size: 96 reactions
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Description

The PARP15 Chemiluminescent Assay Kit is designed to measure the activity of PARP15 (poly-(ADP-ribose) polymerase 15) for screening and profiling applications. The PARP15 assay kit comes in a convenient 96-well format, with enough recombinant purified PARP15 enzyme (amino acids 221-444), reaction substrates, and PARP assay buffer for 100 enzyme reactions.

PARP15 Chemiluminescent Assay Kit

Figure 1. PARP15 Chemiluminescent Assay Kit schematic.
Histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP15 enzyme in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the PARP15 activity.

Need us to run inhibitor screens or profile your compounds against PARP15? Check out our PARP/PARPTrap™ Screening Services or DNA Replication and Repair Screening Services.

Synonyms
poly (ADP-ribose) polymerase 15, poly(ADP-ribosyl)transferase 15, PARP15, PARP-15
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Materials Required But Not Supplied
  • 1x PBS (Phosphate Buffer Saline) Buffer
  • PBST Buffer (1x PBS, containing 0.05% Tween-20)
  • Luminometer or microplate reader capable of reading chemiluminescence
  • Adjustable micropipettor and sterile tips
  • Rotating or rocker platform
Format
Catalog # Name Amount Storage
80517 PARP15, GST-Tag* 1.5 µg -80°C
52029 5x Histone Mixture 1 ml -80°C
78371 PARP Substrate Mixture 2 250 µl -80°C
80602 10x PARP Assay Buffer 1 ml -20°C
79743 Blocking Buffer 3 25 ml +4°C
  0.5 M DTT 200 µl -20°C
80611 Streptavidin-HRP 100 µl +4°C
79670 ELISA ECL Substrate A (translucent bottle) 6 ml Room Temp
ELISA ECL Substrate B (brown bottle) 6 ml Room Temp
79837 96-well module plate 1 Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #
Q460N3
Background

PARP15, also known as poly-(ADP-ribose) polymerase 15 or ARTD7 (diphteria toxin like ADP-ribosyl transferase 7), is part of the PARP family.  ADP ribosylation, which is the addition of an ADP-ribose to a protein, is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Mono-ADP-ribosylation (termed MARylation) is the addition of a unit of ADP-ribose. PARP15 is involved in telomere maintenance, stress response, transcription and immune signaling. Little is known about PARP15, although some inhibitors have been identified. A deeper understanding of the function of this protein in health and disease will benefit the overall oncology field and may bring to light new treatment options.

References

Zhou X., et al., 2022 Biochemical and Biophysical Research Communications 622: 93-100.