TNKS2 Histone Ribosylation Assay Kit (Antibody Detection) (Discontinued)
The TNKS2 Histone Ribosylation Assay Kit (Antibody Detection) is designed to measure Tankyrase-2 activity for screening and profiling applications. The key to the TNKS2 Histone Ribosylation Assay Kit (Antibody Detection) is a highly specific antibody that recognizes poly (ADP-ribose)-modified histone. After coating the plate with the histone mixture, only three simple steps are required. First, NAD+ is incubated with a sample containing assay buffer and enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
This product has been cited 2 times.
PBS buffer
PBST buffer (1x PBS, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform
Paper towels
CatalogNumber |
Reagent |
Amount |
Storage |
|
2 µg |
-80°C |
(Avoid freeze/thaw cycles!) |
||
1 ml |
-80°C |
|||
|
NAD+ |
250 µl |
-80°C |
|
1 ml |
-20°C |
|||
52140K |
Primary antibody 11 |
12.5 µl |
-80°C |
|
52130H |
Secondary HRP-labeled antibody 1 |
10 µl |
-80°C |
|
2 x 40 ml |
+4°C |
|||
6 ml each |
+4°C |
|||
|
8-well strip plate module |
1 plate (12 x 8-well strips) |
+4°C |