TNKS1 Histone Ribosylation Colorimetric Assay Kit

Catalog #: 80582
Size: 96 reactions
$535
Description:
The TNKS1 Histone Ribosylation Colorimetric Assay Kit is designed to measure Tankyrase 1 (TNKS1) activity for screening and profiling applications. TNKS1 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the substrate proteins. The key to the TNKS1 Histone Ribosylation Assay is the biotinylated NAD+ substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated NAD+substrate is incubated with an assay buffer that contains the TNKS1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.
Synonym(s):
TNKS1, poly(ADP-ribose)polymerase 5a, poly(ADP-ribosyl)transferase 5a, PARP5A, TRF1-interacting ankyrin-related ADP-ribose polymerase 1, TANK1, tanykyrase-1
Supplied As:
The TNKS1 assay kit comes in a convenient 96-well format, with purified TNKS1 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.
Format:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

Application Reference(s):
1. Small Molecules Inspired by the Natural Product Withanolides as Potent Inhibitors of Wnt Signaling (2017)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PARP
Product Type:
Assay Kits
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Product Datasheets

80582.pdf