TNKS1 Histone Ribosylation Colorimetric Assay Kit (Discontinued)
The TNKS1 Histone Ribosylation Colorimetric Assay Kit is designed to measure Tankyrase 1 (TNKS1) activity for screening and profiling applications. TNKS1 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the substrate proteins. The key to the TNKS1 Histone Ribosylation Assay is the biotinylated NAD+ substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated NAD+substrate is incubated with an assay buffer that contains the TNKS1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.
This product has been cited 3 times.
| Catalog Number | Reagent | Amount | Storage | |
| 80504 | TNKS1 | 5 μg | -80°C |
|
| 52029 | 5x histone mixture | 1 ml | -80°C | |
| 80601 | 10x Assay Mixture containing biotinylated substrate | 300 μl | -80°C | |
| 80602 | 10x PARP assay buffer | 1 ml | -20°C | |
| 79743 | Blocking buffer 3 | 25 ml | +4°C | |
| 80611 | Streptavidin-HRP | 100 μl | +4°C | |
| Colorimetric HRP substrate | 10 ml | +4°C | ||
| 79964 | Transparent 96-well plate | 1 | Room Temp | |
Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.
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