TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) (Discontinued)

Catalog #

80579

$1,245 *

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Description

The TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) is designed to measure Tankyrase 1 (TNKS1) activity for screening and profiling applications. The key to the TNKS1 Histone Ribosylation Assay (Biotin-labeled NAD+) is the biotinylated substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 384-well plate. Next, the biotinylated TNKS1 substrate is incubated with an assay buffer that contains the TNKS1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

This product has been cited 5 times.

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Synonyms

Tankyrase1, TNKS1, poly (ADP-ribose) polymerase 5a, poly(ADP-ribosyl)transferase 5a, PARP5a

Product Info

Storage and Usage

Citations5

Assay Kit Format

Chemiluminescent

Supplied As

The TNKS1 assay kit comes in a convenient 384-well format, with purified TNKS1 enzyme, histone mixture, and PARP assay buffer for 384 enzyme reactions.

Materials Required But Not Supplied

1x PBS buffer PBST buffer (1x PBS, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog Number	Component	Amount	Storage
80504	TNKS1	2 x 5 μg	-80°C
52029	5x histone mixture	2 x 1 ml	-80°C
80601	10x Assay Mixture Containing Biotinylated Substrate	2 x 300 μl	-80°C
80602	10x PARP assay buffer	2 x 1 ml	-20°C
79743	Blocking buffer 3	2 x 25 ml	+4°C
80611	Streptavidin-HRP	2 x 100 μl	+4°C
79670	ELISA ECL substrate A (translucent bottle)	2 x 6 ml	Room Temp
79670	ELISA ECL substrate B (brown bottle)	2 x 6 ml	Room Temp
	384-well plate	1	Room Temp.

UniProt #

O95271

Background

TNKS1 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the substrate proteins.

References

Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

Storage/Stability

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

Scientific Category

PARP

Regulatory Status

Research Use Only

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TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) (Discontinued)

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