TNKS1 Histone Ribosylation Assay Kit (Antibody Detection) (Discontinued)

Catalog #
80574
$1,135 *
Catalog number 80574, TNKS1 Histone Ribosylation Assay Kit (Antibody Detection) (Discontinued), has been discontinued. Please find our suggested alternative here.
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Description

The TNKS1 Histone Ribosylation Assay Kit (Antibody Detection) is designed to measure Tankyrase-1 activity for screening and profiling applications. The key to the TNKS1 Histone Ribosylation Assay Kit (Antibody Detection) is a highly specific antibody that recognizes poly (ADP-ribose)-modified histone. After coating the plate with the histone mixture, only three simple steps are required. First, NAD+ is incubated with a sample containing assay buffer and enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Synonyms
Tankyrase1, TNKS1, poly (ADP-ribose) polymerase 5a, poly(ADP-ribosyl)transferase 5a, PARP5a
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Supplied As
The TNKS1 Histone Ribosylation Assay Kit (Antibody Detection) comes in a convenient format, with 8-well strips, histone mixture substrate, antibody against poly (ADP-ribose) modified histone, the secondary HRP-labeled antibody, NAD+, PARP assay buffer, an
Materials Required But Not Supplied

PBS buffer
PBST buffer (1x PBS, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog
Number

Reagent

Amount

Storage

80504

TNKS1

2 µg

-80°C

(Avoid freeze/thaw cycles!)

52029

5× histone mixture

1 ml

-80°C

 

NAD+

250 µl

-80°C

80602

10x PARP Assay Buffer

1 ml

-20°C

52140K

Primary antibody 11

12.5 µl

-80°C

52130H

Secondary HRP-labeled antibody 1

10 µl

-80°C

79743

Blocking Buffer 3

2 x 40 ml

+4°C

79670

ELISA ECL substrate (2 components)

6 ml each

+4°C

 

8-well strip plate module

1 plate

(12 x 8-well strips)

+4°C

UniProt #
O95271
References
Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.