PARP11 Chemiluminescent Assay Kit
The PARP11 Chemiluminescent Assay Kit is designed to measure the activity of PARP11 (poly-(ADP-ribose) polymerase 11) for screening and profiling applications. The PARP11 assay kit comes in a convenient 96-well format, with enough recombinant purified PARP11 enzyme (amino acids 8-338), reaction substrate, and PARP assay buffer for 100 enzyme reactions.
Figure 1. PARP11 Chemiluminescent Assay Kit schematic.
Histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP11 enzyme in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the PARP11 activity.
Need us to run inhibitor screens or profile your compounds against PARP11? Check out our PARP/PARPTrap™ Screening Services or DNA Replication and Repair Screening Services.
This product has been cited 3 times.
- 1x PBS (Phosphate Buffer Saline) Buffer
- PBST Buffer (1x PBS, containing 0.05% Tween-20)
- Luminometer or microplate reader capable of reading chemiluminescence
- Adjustable micropipettor and sterile tips
- Rotating or rocker platform
| Catalog # | Name | Amount | Storage |
| 80511 | PARP11, GST-Tag, His-Tag* | 32 µg | -80°C |
| 52029 | 5x Histone Mixture | 1 ml | -80°C |
| 78371 | PARP Substrate Mixture 2 | 4 x 250 µl | -80°C |
| 80602 | 10x PARP Assay Buffer | 1 ml | -20°C |
| 79743 | Blocking Buffer 3 | 25 ml | +4°C |
| 0.5 M DTT | 200 µl | -20°C | |
| 80611 | Streptavidin-HRP | 100 µl | +4°C |
| 79670 | ELISA ECL Substrate A (translucent bottle) | 6 ml | Room Temp |
| ELISA ECL Substrate B (brown bottle) | 6 ml | Room Temp | |
| 79837 | 96-well module plate | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube
PARP11, also known as poly-(ADP-ribose) polymerase 11 or ARTD11 (ADP-Ribosyltransferase Diphtheria Toxin-Like 11), is part of the PARP family. ADP ribosylation, which is the addition of an ADP-ribose to a protein, is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Mono-ADP-ribosylation (termed MARylation) is the addition of a unit of ADP-ribose. PARP11 is involved in the regulation of IFN-I (interferon I) receptor, IFNAR1, by performing MARylation on β-TrCP (β-transducin repeat-containing protein), which acts as an E3 ligase and ubiquitinates IFNAR1. The downregulation of IFNAR1 in CTLs (cytotoxic T lymphocytes) occurs when an immunosuppressive TME (tumor microenvironment) is present, in a process mediated by PARP11. Ablation of PARP11 can counteract this phenomenon, and the use of CTLs with a CAR (chimeric antigen receptor) that was engineered to inactivate PARP11 resulted in a higher efficacy in solid tumors. Targeting PARP11 genetically or pharmacologically may thus provide new therapeutic avenues in the treatment of solid tumors.
Zhang H., et al., 2022 Nature Cancer 3: 808-820.
Munoz I. and Beavis P., 2022 Nature Cancer 3: 790-792.
Brooks D., et al., 2023 Current Opinion in Chemical Biology 77:102402.
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