PARP10 Chemiluminescent Assay Kit
The PARP10 Chemiluminescent Assay Kit is designed to measure the activity of PARP10 (poly-(ADP-ribose) polymerase 10) for screening and profiling applications. The PARP10 assay kit comes in a convenient 96-well format, with enough recombinant purified PARP10 enzyme, reaction substrates, and PARP assay buffer for 100 enzyme reactions.
Figure 1. PARP10 Chemiluminescent Assay Kit schematic.
Histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP10 enzyme in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to PARP10 activity.
Need us to run inhibitor screens or profile your compounds against PARP10? Check out our PARP/PARPTrap™ Screening Services or DNA Replication and Repair Screening Services.
This product has been cited 1 time.
- 1x PBS (Phosphate Buffer Saline) Buffer
- PBST Buffer (1x PBS, containing 0.05% Tween-20)
- Luminometer or microplate reader capable of reading chemiluminescence
- Adjustable micropipettor and sterile tips
- Rotating or rocker platform
| Catalog # | Name | Amount | Storage |
| 80510 | PARP10* | 2 µg | -80°C |
| 52029 | 5x Histone Mixture | 1 ml | -80°C |
| 78371 | PARP Substrate Mixture 2 | 2 x 250 µl | -80°C |
| 80602 | 10x PARP Assay Buffer | 1 ml | -20°C |
| 79743 | Blocking Buffer 3 | 25 ml | +4°C |
| 0.5 M DTT | 200 µl | -20°C | |
| 80611 | Streptavidin-HRP | 100 µl | +4°C |
| 79670 | ELISA ECL Substrate A (translucent bottle) | 6 ml | Room Temp |
| ELISA ECL Substrate B (brown bottle) | 6 ml | Room Temp | |
| 79837 | 96-well module plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube
PARP10, also known as poly-(ADP-ribose) polymerase 10, NAD+ ADP-ribosyltransferase 10 and ARTD10, is part of the PARP family. ADP ribosylation, which is the addition of an ADP-ribose to a protein, is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Mono-ADP-ribosylation (termed MARylation) is the addition of a unit of ADP-ribose. PARP10 is found both in the cytosol and the nucleus, having c-myc, histones, PCNA (proliferating cell nuclear antigen), GSK3β (gyclogen synthase kinase-3 beta) and other proteins as ligands. It is involved in metabolism, with lower levels of PARP10 linking to lower fatty acid oxidation, DNA damage and repair in response to hydroxyurea and UV light and maintenance of genome stability. PARP10 has also been linked to cancer, acting as a tumor suppressor by negatively regulating Aurora A and thus negatively regulating G2/M transition during cell cycle. Inhibition of PARP10 presents itself as a potential new tool in cancer therapy.
Marton J., et al., 2018 PLos One 13(11): e0187789.
Zhao Y., et al., 2018 Oncogene 37(22):2921-2935.
Dhoonmoon A. and Nicolae C., NAR Cancer 5(1):zcad009.
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