PARP1 Colorimetric Assay Kit

Catalog #: 80580
Size: 96 reactions
$510
Description:
The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP1 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.
Synonym(s):
poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1, PARP-1
Supplied As:
The PARP1 assay kit comes in a convenient 96-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 100 enzyme reactions.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

1.Brown, J.A., Marala, R.B. 2002. J. Pharmacol. Toxicol. Methods 47:137-41.

Application Reference(s):
1. Olaparib hydroxamic acid derivatives as dual PARP and HDAC inhibitors for cancer therapy (2017)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PARP
Product Type:
Assay Kits
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Product Datasheets

80580.pdf