TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)
Catalog # 80579
Size: 384 reactions

DESCRIPTION: The TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) is
designed to measure Tankyrase 1 (TNKS1) activity for screening and profiling
applications. TNKS1 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the
substrate proteins. The TNKS1 assay kit comes in a convenient 384-well format, with
purified TNKS1 enzyme, histone mixture, and PARP assay buffer for 384 enzyme
reactions. The key to the TNKS1 Histone Ribosylation Assay (Biotin-labeled NAD+) is
the biotinylated substrate. With this kit, only three simple steps are required for TNKS1
reactions. First, histone proteins are coated on a 384-well plate. Next, the biotinylated
TNKS1 substrate is incubated with an assay buffer that contains the TNKS1 enzyme.
Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP
substrate to produce chemiluminescence that can be measured using a
chemiluminescence reader.

COMPONENTS:
 

STABILITY: Up to 1 year when stored as recommended.

APPLICATIONS: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.

REFERENCE: Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

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