PARP(s)

 

Poly (ADP-ribose) polymerase (PARP) is a family of proteins that, generally speaking, transfer ADP-ribose units from NAD+ onto target nuclear proteins, forming long branched Poly ADP-ribose (PAR) chains. PARPs play key signaling roles in apoptosis, DNA excision and repair, and chromatin structure. PARPs are also involved in transcriptional regulation of several signaling pathways, including genes involved in inflammation.

Some of the PARP proteins are known as tankyrases (PARP5A and 5B). In particular, tankyrases (TNKS) play a role in the maintenance of telomere length, sister telomere association, and mitotic spindle organization. Tankyrase 1 also destabilizes axin, thereby activating Wnt signaling and driving tumor cell proliferation. Thus, the PARPs, and particularly the TNKS have become important cancer drug targets. Further evidence for this comes from the fact that PARP is overexpressed in lung, ovarian, and breast cancer, and PARP inhibitors are showing dramatic therapeutic potential for breast and ovarian cancer in phase I and II clinical trials.

In addition to the role of TNKS in cancer, PARPs participate in a number of other normal and disease-associated biological processes. PARP1 binds DNMT1 and indirectly regulates DNA methylation. PARP also remodels chromatin by PARylating histones and relaxing chromatin structure. PARP2 is a substrate for histone acetyltransferases PCAF and GCN5L. PARP7 plays a role in adaptive responses to chemical exposure and mediates certain effects of the chemicals. Mice deficient in the PARP DNA repair enzyme are refractory to toxin-induced death of pancreatic beta cells and do not become diabetic. Finally, cleavage of PARP1 by caspases 3 and 7 is considered one of the classic markers of apoptosis. PARP directly induces caspase-independent apoptosis, via the production of PAR, which stimulates mitochondria to release AIF.

BPS is the only company to offer an extensive portfolio of PARP isozymes. Additionally, we offer assay kits, inhibitors, and recombinant histone substrates. Screening and profiling services are also available for over 10 different PARPs.

References:

1.    Ame, J.C., et al., BioEssays.2004; 26(8): 882-893.
2.    Rouleau, M., et al., Nat. Rev. Canc. 2010; 10: 293-301.

 

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Catalog#SizeProduct NamePrice
80551 96 reactions PARP1 Chemiluminescent Assay Kit
$510
80569 384 reactions PARP1 Chemiluminescent Assay Kit (384-well)
$1,050
80560 96 reactions PARP10 Chemiluminescent Assay Kit
$995
80561 96 reactions PARP11 Chemiluminescent Assay Kit
$995
80568 32 reactions PARP14 Chemiluminescent Assay Kit
$975
80567 96 reactions PARP15 Chemiluminescent Assay Kit
$995
80552 96 reactions PARP2 Chemiluminescent Assay Kit
$650
80702 384 reactions PARP2 Homogeneous Assay Kit
$1,800
80553 96 reactions PARP3 Chemiluminescent Assay Kit
$900
80556 32 reactions PARP6 Chemiluminescent Assay Kit
$975
80575 384 reactions TNKS1 Histone Ribosylation Assay Kit (Antibody Detection)
$1,850
80574 96 reactions TNKS1 Histone Ribosylation Assay Kit (Antibody Detection)
$975
80573 96 reactions TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)
$510
80579 384 reactions TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)
$1,025
80577 384 reactions TNKS2 Histone Ribosylation Assay Kit (Antibody Detection)
$1,850
80576 96 reactions TNKS2 Histone Ribosylation Assay Kit (Antibody Detection)
$975
80572 384 reactions TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)
$1,425
80578 96 reactions TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)
$715
27310 25 mg 1,5-Isoquinolinediol
$75
80602 1 ml 10x PARP Assay Buffer
$30
80601 300 ┬Ál 10x PARP Assay Mixture
$220
27601-1 5 mg A-966492
$170
27601-2 10 mg A-966492
$250
27601-3 50 mg A-966492
$690
27101 5 mg ABT-888 (Veliparib)
$190

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