PLK1, His-tag Recombinant

Catalog #

40033

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human Plk1 also known as STPK13 and polo-like kinase 1, GenBank Accession No. NM_005030, a.a. full length with C-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=69 kDa.

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Synonyms

STPK13, polo-like kinase 1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥83%

Supplied As

Aqueous buffer solution.

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 250 mM Imidazole.

69 kDa

Amino Acids

full length

Specific Activity

≥14 pmol/min/µg

Genbank #

NM_005030

UniProt #

P53350

Tag(s)

C-terminal His-tag

Background

PLK1 is a member of the Polo-Like Kinase family that localize to centrosomes or spindle pole bodies and undergo dramatic subcellular relocation during the cell cycle. Deregulated activities of PLKs often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics. PLKs also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies, and ectopic expression of PLK1 dominant negative protein leads to rapid cell death.

References

1. Fabbro, M. et al., Dev. Cell 9 (4), 477-488. (2005).
2. Liu, X., et al., Mol. Cell. Biol. 25, 4993-5010 (2005).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 1 mg/ml Casein substrate and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40033 Lot 170315.pdf

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