PKM1, His-tag

Catalog #: 40502
Size: 20 µg
$455
Species: Human
Host Species: E. coli
MW: 59 kDa
Genbank #: NM_182470
Tag(s): N-terminal His-tag
a.a: full length
Background:
PKM1 and PKM2 are different splicing products of the PKM gene (exon 9 for PKM1 and exon 10 for PKM2). The M1 isoform (PKM1) is expressed in muscle and brain, and the M2 isoform is expressed during embryogenesis, in adipose tissue and pancreatic islets, and is the predominant form found in cancer cells.
Description:
Human pyruvate kinase muscle (PKM1), GenBank Accession No. NM_182470, full length with N-terminal His-tag, MW=59 kDa, expressed in an E. coli expression system.
UniProt
P14618
Synonym(s):
Pyruvate Kinase muscle, PKM1
Specific Activity:
≥54 pmol/min/µg
Assay Conditions:
A 50 µl reaction is conducted in a buffer containing 50 mM Tris (pH 7.4), 10 mM MgCl2, 50 mM KCl, 2 mM ADP, 10 mM phosphoenolpyruvate (PEP) at room temperature for 15 min. ATP production is detected using Kinase-Glo® Luminescent Kinase Assay Platform.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol, and 3 mM DTT
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Parkison C. et al., Biochem Biophys Res Commun. 179(1) ; 668-74 (1991).
2. Bluemlein K. et al., Oncotarget. 2011 May 22.

Application Reference(s):
1. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival (2017)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes-Kinase
Product Type:
Recombinant Protein
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