PKM1 (PKM2/Variant 2), His-Tag Recombinant

Catalog #
40502
$495 *
Size: 20 µg
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Description

Recombinant  human PKM2/Variant 2 (human pyruvate kinase muscle), full length encompassing amino acids 2-531(end). This construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

This product has been cited 2 times.

Synonyms
Pyruvate Kinase muscle, PKM1
Product Info
Storage and Usage
Citations2
Species
Human
Construct
PKM1(PKM2/Variant 2) (His-2-531(end))
Host Species/Expression System
E. coli
Purity

≥90%

Format
Aqueous buffer solution
Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT, variable imidazole

MW
59 kDa
Amino Acids
2-531(end)
Specific Activity
≥54 pmol/min/µg
Genbank #
NM_182470
UniProt #
P14618
Tag(s)
N-terminal His-tag
Background
PKM1 and PKM2 are different splicing products of the PKM gene (exon 9 for PKM1 and exon 10 for PKM2). The M1 isoform (PKM1) is expressed in muscle and brain, and the M2 isoform is expressed during embryogenesis, in adipose tissue and pancreatic islets, and is the predominant form found in cancer cells.
References

1. Parkison C. et al., Biochem Biophys Res Commun. 179(1) ; 668-74 (1991).
2. Bluemlein K. et al., Oncotarget. 2011 May 22.