PKCα (PKCalpha), GST-tag Recombinant

Catalog #

40157

$330 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human PKCα (PKCalpha), full length, GenBank Accession No. NM_002737, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system. MW= 102 kDa.

This product has been cited 1 time.

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Synonyms

AAG6, PKCA, PRKCA, PRKACA, MGC129900, MGC129901, PKCα protein kinase c alpha

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 3 mM DTT, 20% glycerol, 1.6 mM Glutathione

102 kDa

Amino Acids

full length

Specific Activity

320 pmol/min/µg

Genbank #

NM_002737

UniProt #

P17252

Tag(s)

N-terminal GST-tag

Background

PKCα (PKC alpha) is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC-alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control(1). PKCα has been assigned to the chromosome region 17q22-q23.2 and has been identified as a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes (2).

References

1. Coussens, L. et al. Science 233: 859-866, 1986.
2. Braz, J. C. et al. Nature Med. 10: 248-254, 2004.

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.

Assay Conditions

Assay was done in Kinase buffer containing 0.1 mM CaCl2 and lipid activator (1 mg/ml Phosphatidyl Serine and 0.02 mg/ml Diacylglycerol in 0.3% TritonX-100) using Histone H1 (0.1 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40157 Lot 170329-3

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PKCα (PKCalpha), GST-tag Recombinant

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