PI3 kinase (p120γ), His-tag Recombinant

Catalog #

40625

$435 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Human p120γ (gamma) (GenBank Accession No. NM_002649), full length with N-terminal His-tag, MW=130 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 2 times.

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Synonyms

PI3CG, 3-kinase catalytic subunit gamma isoform; p120-PI3K; PI3K-gamma, p120γ

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

P120γ Specific Activity

Product Info

Storage and Usage

Citations2

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 3 mM DTT, and 50% glycerol

130 kDa

Amino Acids

full length

Specific Activity

1200 pmol/min/μg.

Genbank #

NM_002649

UniProt #

P48736

Tag(s)

N-terminal His-tag

References

1. Webb, LM., et al., J Immunol. 175(5):2783-7 (2005).
2. Voigt, P., et al., J Biol Chem. 280(6):5121-7(2005).

Storage/Stability

>6 months at –80°C

Assay Conditions

A 25 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl₂, 100 uM ATP, 200 μM PI:PS substrate (BPS catalog #40560) and 0-200 ng p120γ enzyme for 15 minutes at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform (Promega Corporation, Madison, WI).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Lipids

Regulatory Status

Research Use Only

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Datasheets/Protocols

40625_Lot110922.pdf 40625_Lot120410-G2.pdf 40625_Lot100727.pdf

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PI3 kinase (p120γ), His-tag Recombinant

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