PI3 kinase (p120γ), His-tag

Catalog #: 40625
Size: 20 µg
$405
Species: Human
Host Species: Sf9 insect cells
MW: 130 kDa
Genbank #: NM_002649
Tag(s): N-terminal His-tag
a.a: full length
Description:
Human p120γ (gamma) (GenBank Accession No. NM_002649), full length with N-terminal His-tag, MW=130 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
P48736
Synonym(s):
PI3CG, 3-kinase catalytic subunit gamma isoform; p120-PI3K; PI3K-gamma, p120γ
Specific Activity:
1200 pmol/min/μg.
Assay Conditions:
A 25 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl2, 100 uM ATP, 200 μM PI:PS substrate (BPS catalog #40560) and 0-200 ng p120γ enzyme for 15 minutes at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform (Promega Corporation, Madison, WI).
Formulation:
25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 3 mM DTT, and 50% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Webb, LM., et al., J Immunol. 175(5):2783-7 (2005).
2. Voigt, P., et al., J Biol Chem. 280(6):5121-7(2005).

Application Reference:
Discovery of a potent and isoform-selective targeted covalent inhibitor of the lipid kinase PI3Kα (2013)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Lipids
Product Type:
Recombinant Protein
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