PDE7B-HEK293 Recombinant Cell Line

Catalog #
60412
$9,270 *
Size: 2 vials
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Description

Recombinant HEK293 cell line expressing human PDE7B (phosphodiesterase 7B, accession number NM_018945).

Human PDE7B has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7B expression was confirmed by Western blotting. The function of expressed PDE7B was validated by cAMP detection assay. The regulation of intracellular level of cAMP by PDE7B in PDE7B stably-expressed HEK293cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains the luciferase gene that is under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells inducing the expression of the luciferase reporter whereas hPDE7B-HEK293 cells showed reduction in the level of cAMP that resulted in lowered expression of luciferase.

This product has been cited 1 time.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
PDE7B, phosphodiesterase 7B, PDE7-B, PDE
Product Info
Storage and Usage
Citations1
Host Cell Line
HEK293 (human)
Species
Human
Supplied As
Each vial contains ~1 X 106 cells in 1 ml of 10% DMSO.
Format
aqueous solution containing DMSO
Genbank #
NM_018945
UniProt #
Q9NP56
Mycoplasma Testing
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Background
Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP by hydrolyzing cAMP and cGMP to their inactive 5' monophosphates. These cyclic nucleotides play an important role as second messengers in diverse physiological functions. PDE7 is a cAMP-specific enzyme and two PDE genes (PDE7A and PDE7B) have been identified. PDE7 is widely expressed in various tissues, with PDE7A found primarily in skeletal muscle, T lymphocytes, and pancreas, while high levels of PDE7B are detected in brain, heart, and liver. Inhibition of PDE7 activity by its inhibitors leads to elevated intracellular level of cAMP.
References
1. Malik, R. et al. (2008) Cloning, stable expression of human phosphodiesterase 7A and development of an assay for screening of PDE7 selective inhibitors. Appl. Microbiol. Biotechnol. 77 (5): 1167-1173
2. Fan Chung, K. (2006) Phosphodiesterase inhibitors in airways disease. Eur. J. Pharmacol. 533(1-3):110-117