Mouse PDE7B, GST-tag

Catalog #: 60073
Size: 10 µg
$425
Species: Mouse
Host Species: Sf9 insect cells
MW: 65 kDa
Genbank #: NM_013875
Tag(s): N-terminal GST-tag
a.a: 108–446(end)
Description:
Mouse PDE7B (GenBank Accession No. NM_013875), a.a. 108-446(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
UniProt
Q9NP56
Synonym(s):
PDE7B, phosphodiesterase 7B
Specific Activity:
≥240 pmol/min/µg.
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
Assayed using BPS's PDE7B kit, Cat. # 60371. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of mPDE7B at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Gardner, C., et al. (2000). Biochem Biophys Res Commun. May 27;272(1):186-92.
2. Reyes-Irisarri, E., et al. (2005). Neuroscience 132(4):1173-85.
3. Zhang, L., et al. (2008). Proc. Natl. Acad. Sci. USA Dec 9;105(49):19532-7.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
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Product Datasheets

60073_Lot100915.pdf