Mouse PDE5A1, GST-tag

Catalog #: 60051
Size: 5 µg
$425
Species: Mouse
Host Species: Sf9 insect cells
MW: 124 kDa
Genbank #: NM_153422
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Mouse PDE5A (GenBank Accession No. NM_153422), full length, with N-terminal GST-tag, MW=124 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q8CG03
Synonym(s):
PDE5A, phosphodiesterase 5A
Specific Activity:
≥ 4630 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 0.1 mg/ml BSA, 200 µM cGMP, 2.5 kU 5'-nucleotidase, and serial dilutions of mPDE5A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -GMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18 mM glutathione, 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Das, A. et al., J. Biol. Chem. 280 (13), 12944-12955 (2005).
2. Champion, H.C.et al., Proc. Natl. Acad. Sci. U.S.A. 102 (5), 1661-1666 (2005).
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
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60051_Lot80207.pdf