Dog PDE4D, GST-tag

Catalog #: 60056
Size: 5 µg
$440
Species: Dog
Host Species: Sf9 insect cells
MW: 104 kDa
Genbank #: XP_003433853
Tag(s): N-terminal GST-tag
a.a: 2–679 (end)
Description:
Dog phosphodiesterase 4D (PDE4D) GenBank Accession No. XP_003433853, a.a. 2-679 (end), with N-terminal GST-tag, MW 104 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
UniProt
F1PKQ9
Synonym(s):
PDE4D, phosphodiesterase 4D
Specific Activity:
≥ 5665 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C.
Assay Conditions:

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of dPDE4D at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Formulation:
40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT
Format:
Aqueous buffer solution
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Lynex, C.N., et al., Cell Signal. 2008 Dec,20(12):2247-55.
2. Smith, C.J., et al., Circulation. 1997 Nov 4,96(9):36116-23.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

60056_Lot130718-G1.pdf