Dog PDE4B, GST-tag

Catalog #: 60055
Size: 5 µg
$440
Species: Dog
Host Species: Sf9 insect cells
MW: 110 kDa
Genbank #: XP_003434692
Tag(s): N-terminal GST-tag
a.a: 2–737(end)
Description:
Dog phosphodiesterase 4B (PDE4B) GenBank Accession No. XP_003434692, a.a. 2-737(end), with N-terminal GST-tag, MW 110 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
UniProt
F1PJ12
Synonym(s):
PDE4B, phosphodiesterase 4B
Specific Activity:
≥ 3882 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C.
Assay Conditions:

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of dPDE4B at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Formulation:
40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT
Format:
Aqueous buffer solution
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Girgenti, M.J., et al., PLoS One 2012,7(2):e32404.
2. Zhao, H., et al., Eur. J. Pharmacol. 2008 Aug 20,590(1-3):29-35.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60055_Lot130717-G1.pdf