PDE3A, (669-end), His-GST-tags

Catalog #: 60032
Size: 5 µg
$425
Species: Human
Host Species: Sf9 insect cells
MW: 82 kDa
Genbank #: NM_000921
Tag(s): N-terminal His-GST-tags
a.a: 669–1141(end)
Description:
Human PDE3A (GenBank Accession No. NM_000921), a.a. 669-1141(end) with N-terminal His-GST-tag, MW = 82 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q14432
Synonym(s):
PDE3A, phosphodiesterase 3A
Specific Activity:
≥ 4000 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
Assayed using BPS's PDE3A Assay Kit, Cat. # 60330. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE3A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
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