PDE2A, GST-Th-Tag

Catalog #: 60020
Size: 5 µg
$425
Species: Human
Host Species: Sf9 insect cells
MW: 132 kDa
Genbank #: NM_002599
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human PDE2A1 (GenBank Accession No. NM_002599), full length, with N-terminal GST-tag and N-terminal thrombin cleavage site, MW=132 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
O00408
Synonym(s):
PDE2A, phosphodiesterase 2A
Specific Activity:
≥ 3442 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
Assayed using BPS's PDE2A Assay Kit, Cat. # 60320. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE2A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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