Mouse PDE1C, GST-tag

Catalog #: 60012
Size: 10 µg
$425
Species: Mouse
Host Species: Sf9 insect cells
MW: 100 kDa
Genbank #: NM_001025568
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Mouse PDE1C (GenBank Accession No. NM_001025568), full length with N-terminal GST-tag, MW= 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q64338
Synonym(s):
PDE1C, phosphodiesterase 1C
Specific Activity:
≥ 16,650 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
Assayed using BPS's Mouse PDE1C Assay Kit, Cat. # 61312. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE1C at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Gong S. et al., Nature 425 (6961), 917-925 (2003).
2. Spence S. et al., Biochem. J. 321 (PT 1), 157- 163 (1997).
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
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Product Datasheets

60012_Lot130430.pdf