PDE1C, GST-tag

Catalog #: 60013
Size: 10 µg
$425
Species: Human
Host Species: Sf9 insect cells
MW: 98 kDa
Genbank #: NM_005020
Tag(s): N-terminal GST-tag
a.a: 2–634(end)
Description:
Human PDE1C (GenBank Accession No. NM_005020), a.a. 2-634(end), with N-terminal GST-tag, MW=98 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
UniProt
Q14123
Synonym(s):
PDE1C, phosphodiesterase 1C
Specific Activity:
≥ 15,000 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
Assayed using BPS's PDE1C Assay Kit, Cat. # 60312. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE1C at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween-20, and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Vandeput, F., et al. (2007). J. Biol. Chem. Nov 9;282(45):32749-57.
2. Cygnar, K.D., Zhao, H. (2009). Nat. Neurosci. Apr;12(4):454-62.
3. Ortiz-Capisano, M.C., et al. (2009). Am. J. Physiol. Regul. Integr. Comp. Physiol. Nov;297(5):R1469-76.

Application Reference(s):
1. Identification and characterization of a potent and biologically-active PDE4/7 inhibitor via fission yeast-based assays (2017)
2. A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
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Product Datasheets

60013_Lot130104-G.pdf