PDE7A Assay Kit

Catalog #
60370
$405 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The PDE7A Assay Kit is designed for identification of PDE7A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE7A to the binding agent. The key to the PDE7A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE7A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE7A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.

Need us to run inhibitor screens or profile your compounds against PDE7A? Check out our Phosphodiesterase Screening Services.

This product has been cited 2 times.

Synonyms
inhibitor screening, assay kit, PDE7A, PDE7-A, PDE
Product Info
Storage and Usage
Citations2
Assay Kit Format
Fluorescence Polarization
Supplied As
The PDE7A inhibitor screening assay kit comes in a convenient 96-well format,including purified PDE7A enzyme, fluorescently labeled PDE7A substrate (cAMP),binding agent, and PDE assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied

Fluorescent microplate reader capable to measure fluorescence polarization

Format
Catalog
Number
Component Amount Storage 
60070 PDE7A recombinant enzyme 1 µg -80°C


Avoid

freeze/
thaw
cycles!
60200 FAM-Cyclic-3′, 5′-AMP: 20 µM 50 µl -80°C
60393 PDE assay buffer 25 ml -20°C
60390 Binding Agent 100 µl +4°C
60391 Binding Agent Diluent (cAMP) 10 ml +4°C
79685 Black, low binding, microtiter plate 1 Room Temp.
UniProt #
Q13946-2
Background
Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE7A is widely expressed in various tissues including skeletal muscle, T lymphocytes, brain and pancreas and plays and important role in the regulation of osteoblastic differentiation. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.