PDE4B2 Assay Kit

Catalog #: 60343
Size: 96 reactions
Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE4 selective inhibitors are currently in clinical trials for the treatment of diseases related to inflammatory disorders. Increased expression of PDE4B2 was observed in the near-term myometrium. PDE4B2 can be induced by its own substrate, under the control of one of the major utero-contractile agonist, PGE2. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.
The PDE4B2 Assay Kit is designed for identification of inhibitors of PDE4B2 using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4B2 to the binding agent. The key to the PDE4B2 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4B2 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4B2 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.
inhibitor screening, assay kit, PDE4B
Supplied As:
The PDE4B2 Assay Kit comes in a convenient 96-well format, with purified PDE4B2enzyme, fluorescently labeled PDE4B2 substrate (cAMP), binding agent, and PDE assaybuffer for 100 enzyme reactions.
DMSO >1%, strong acids or bases, ionic detergents, high salt

Assay Kit Components

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. Cullen MD, et al. Bioorg Med Chem Lett. 2008; 18(4):1530-3.
2. Mé hats C, et al. BMC Pregnancy Childbirth. 2007; 7 Suppl 1:S12.

Application Reference(s):
Structure based virtual screening to identify selective phosphodiesterase 4B inhibitors (2015)

Fluorescent microplate reader capable to measure fluorescence polarization
Avoid freeze/thaw cycles
Scientific Category:
Product Type:
Assay Kit
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