Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. Mouse PDE5A, also known as cGMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility. The Mouse PDE5A1 Assay Kit is designed for identification of inhibitors of Mouse PDE5A1 using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE5A1 to the binding agent. The Fluorescence Polarization signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.
The Mouse PDE5A1 Assay Kit comes in a convenient 96-well format, with purified Mouse PDE5A1 enzyme, fluorescently labeled substrate (cGMP), binding agent, and PDE assay buffer for 100 enzyme reactions.
Format
COMPONENTS:
References
1. Tommpo, et al., Adv Neurobiol. 2017;17:103-131.
2. Clapcote SJ, et al., NeuroReport. 2007; Nov; 18(17):1841-1844.
Storage/Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol.
Shipping Temperature
-80°C
Warnings
This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) and equipped with the required partsto read the FP signal. For more information FP technology, visit our Tech Note: FP, assay principles and applications.
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
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