PHGDH, His-tag

Catalog #: 71079
Size: 100 µg
$340
Species: Human
Host Species: E. coli
MW: 57.5 kDa
Genbank #: NM_006623
Tag(s): C-terminal His-tag
a.a: 1 – 533 (end)
Description:
Human phosphoglycerate dehydrogenase (PGDH), also known as PDG, a.a. 1 – 533 (end) (GenBank Accession No. NM_006623) with C-terminal His-tag, MW = 57.5 kDa, expressed in an E. coli expression system.
UniProt
O43175
Synonym(s):
phosphoglycerate dehydrogenase, PGDH, PDG
Specific Activity:
≥80 pmol/min/µg
Assay Conditions:
The reaction mixture contained 100 mM Tris-HCl, pH 8.8, 400 mM NaCl, 0.2 mM DTT, 2.5 mM 3-phospho-D-glyceric acid (Sigma P8877), 1 mM NAD, and various amount of PHGDH in a final volume of 50 µL. Reactions were initiated with the addition of PHGDH, and the increase in NADH fluorescence (Ex340/Em460 nm) was continuously monitored for 10 min at room temperature.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 170 mM imidazole, 0.04% Tween-20, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Mullarky, E., et al., Pigment Cell Melanoma Res. 2011 Dec;24(6):1112-5.
2. Liu, J., et al., J Neurooncol. 2013 Feb;111(3):245-55.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes-Dehydrogenase
Product Type:
Recombinant Protein
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Product Datasheets

71079_Lot121912.pdf