pf-HDAC1, Malaria, His-tag, FLAG-tag Recombinant

Catalog #

50063

$440 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Malarial (Plasmodium falciparum) HDAC, Genbank Accession No. AAD22407.1, full length (1-450) with C-terminal His-tag and FLAG-tag, MW=53.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 20 times.

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Synonyms

histone deacetylase 1 plasmodium, falciparum, pf-HDAC-1 Malaria

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations20

Species

Malaria (Plasmodium falciparum)

Host Species/Expression System

Sf9 insect cells

Purity

≥58%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol.

53.4 kDa

Amino Acids

1–450

Specific Activity

≥1.85 pmol/min/µg

Genbank #

AAD22407.1

UniProt #

Q7K6A1

Tag(s)

C-terminal His-tag and FLAG-tag

References

1. Chen et al., J. Med. Chem. 2008 Jun 26; 51(12):3437-48.
2. Joshi et al., Mol. Biochem. Parasitol. 1999 Mar 15; 99(1):11-9.

Storage/Stability

>6 months at -80°C.

Assay Conditions

25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and pf-HDAC1. HDAC reaction was done for 30 min at 37°C followed by treatment with 50 µl of HDAC developer (Cat. #50030) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Deacetylase

Regulatory Status

Research Use Only