Peroxiredoxin I, His-tag Recombinant

Catalog #

70010

$535 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human Peroxiredoxin I (GenBank Accession No. NM_002574), full length, with N-terminal His- tag, MW=23 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 1 time.

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Synonyms

PRDX1

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Product Data Gallery

14% SDS-PAGE Coomassie Staining

PRDX1 Specific Activity

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.

23 kDa

Amino Acids

full length

Specific Activity

≥896 pmol/min/µg

Genbank #

NM_002574

UniProt #

Q06830

Tag(s)

N-terminal His-tag

References

1. Maksimov,V. et al. Genetika 42 (2), 274-277 (2006)
2. Jang,H.H et al. FEBS Lett. 580 (1), 351-355 (2006)

Storage/Stability

>6 months at –80°C.

Assay Conditions

1.88 µg of PRDX1 was incubated in 50 mM HEPES (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30°C for 5 min, followed by the addition of 0.22 mM H₂O₂. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of biochemical function, enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Peroxiredoxin

Regulatory Status

Research Use Only

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Datasheets/Protocols

70010_Lot_160622.pdf

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Peroxiredoxin I, His-tag Recombinant

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