PDE7B (Rat), GST-tag Recombinant

Catalog #

60075

$455 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Rat PDE7B (GenBank Accession No. NM_080894), a.a. 108-446(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

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Synonyms

PDE7B, phosphodiesterase 7B, PDE7-B, PDE

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Rat

Host Species/Expression System

Sf9 insect cells

Purity

≥30%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT.

65 kDa

Amino Acids

108–446(end)

Specific Activity

≥324 pmol/min/µg.

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Genbank #

NM_080894

UniProt #

Q8VIE4

Tag(s)

N-terminal GST-tag

References

1. Gardner, C., et al. (2000). Biochem Biophys Res Commun. May 27;272(1):186-92.
2. Reyes-Irisarri, E., et al. (2005). Neuro-science 132(4):1173-85.
3. Zhang, L., et al. (2008). Proc. Natl. Acad. Sci. USA Dec 9;105(49):19532-7.

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of rPDE7B at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only