PDE7B, GST-tag, His-tag

Catalog #: 60071
Size: 10 µg
$425
Species: Human
Host Species: Sf9 insect cells
MW: 66 kDa
Genbank #: NM_018945
Tag(s): N-terminal GST-tag, C-terminal His-tag
a.a: 109–end
Description:
Human PDE7B GenBank Accession No. NM_018945, (a.a. 109-end) with N-terminal GST-tag and C-terminal His-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q9NP56
Synonym(s):
PDE7B, phosphodiesterase 7B
Specific Activity:
≥226 pmol/min/µg.
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
Assayed using BPS's PDE7B kit, Cat. # 60371. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE7B at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
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Product Datasheets

60071_Lot100127.pdf