PDE6C (Mouse), GST-Tag Recombinant

Catalog #

60065

$460 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Mouse PDE6C, GenBank Accession No. NM_033614, 2-861 (end), with N-terminal GST tag, MW = 126 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PDE6, PDE6C, PDE6C Mouse, PDE6-C, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9 insect cells

Purity

≥2%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCL, 10 mM Glutathione, 3 mM DTT and 20% Glycerol

126 kDa

Amino Acids

2-861(end)

Specific Activity

≥ 600 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cGMP to 5′-GMP per min at 37°C.

Genbank #

NM_033614

Tag(s)

N-terminal GST-tag

References

1. Martinez S.E. et al., J Biol Chem. 2008 Sep 19;283(38):25913-9.

2. Stearns G. et al., J Neurosci. 2007 Dec 12; 27(50):13866-74.

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cGMP, and serial dilutions of PDE6C at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C (dry ice)

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only