PDE5A1, GST-Tag Recombinant

Catalog #

60050

$480 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human PDE5A1 (phosphodiesterase 5A), full lengths, encompassing amino acids 1-875(end). This construct contains an N-terminal GST tag. The recombinant protein was affinity purified.

This product has been cited 33 times.

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Synonyms

PDE5A, phosphodiesterase 5A, PDE5-A1, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE5A1 inhibition curves using a fluorescence polarization assay (#60350). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations33

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

40mM Tris pH 8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween-20, 20% glycerol, 3mM DTT

126 kDa

Amino Acids

full length

Specific Activity

≥ 3100 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.

Genbank #

NM_001083

UniProt #

O76074

Tag(s)

N-terminal GST-tag

References

1. Filippi,S. et al., Endocrinology 148 (3), 1019-1029 (2007).
2. Bruder,S. et al., J. Biol. Chem. 281 (29), 19969-19976 (2006).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cGMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE5A1 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -GMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only