PDE4D7, GST-tag

Catalog #: 60047
Size: 5 µg
$440
Species: Human
Host Species: Sf9 insect cells
MW: 111 kDa
Genbank #: NM_001165899
Tag(s): N-terminal GST-tag
a.a: 2–748(end)
Description:
Human PDE4D7 (GenBank Accession No. NM_001165899), a.a. 2-748(end) with N-terminal GST-tag, MW=111 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q08499
Synonym(s):
PDE4D7, phosphodiesterase 4D7
Specific Activity:
≥ 10,000 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4D7 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 20 mM Glutathione and 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Wang, D., et al., (2003). Cell Signal. 15(9):883-91.
2. Houslay, M.D. (2005). Mol Pharmacol. (2005). 68(3):563-7
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
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Product Datasheets

60047 lot 171120-A.pdf