PDE4D2, GST-tag Recombinant

Catalog #

60048

$520 *

Size: 5 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human PDE4D2 (GenBank Accession No. NM_001197221), a.a. 2-507(end) with N-terminal GST tag, MW=84 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 17 times.

●

Synonyms

PDE4D2, phosphodiesterase 4D2, PDE4-D2, PDE

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE4D2 inhibition curves using a fluorescence polarization assay (#60345). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations17

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥86%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol and 16 mM Glutathione.

84 kDa

Amino Acids

2–507(end)

Specific Activity

≥ 13,600 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Genbank #

NM_001197221

UniProt #

Q08499-5

Tag(s)

N-terminal GST-tag

References

1. Fidani, L., et al., Eur. J. Neurol. 14 (7):745-749 (2007).
2. Baillie, G.S., et al., Biochem. J. 404 (1):71-80 (2007).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4D2 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only