PDE4D (Dog), GST-tag Recombinant

Catalog #

60056

$470 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Dog phosphodiesterase 4D (PDE4D) GenBank Accession No. XP_003433853, a.a. 2-679 (end), with N-terminal GST-tag, MW 104 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

This product has been cited 1 time.

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Synonyms

PDE4D, phosphodiesterase 4D, PDE4-D, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations1

Species

Dog

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT

104 kDa

Amino Acids

2–679 (end)

Specific Activity

≥ 5665 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C.

Genbank #

XP_003433853

UniProt #

F1PKQ9

Tag(s)

N-terminal GST-tag

References

1. Lynex, C.N., et al., Cell Signal. 2008 Dec,20(12):2247-55.
2. Smith, C.J., et al., Circulation. 1997 Nov 4,96(9):36116-23.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of dPDE4D at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PDE

Regulatory Status

Research Use Only