PDE4B2, GST-Tag, His-Tag Recombinant

Catalog #

60042

$485 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Human PDE4B2 (GenBank Accession No. NM_001037339), full length, with N-terminal GST-tag and C-terminal His-tag, MW=91.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 8 times.

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Synonyms

PDE4B2, phosphodiesterase 4B2, PDE4-B2, PDE

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

PDE4B2 inhibition curves using a fluorescence polarization assay (#60343). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations8

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween 20, and 20% glycerol.

91.2 kDa

Amino Acids

full length

Specific Activity

≥ 12,300 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Genbank #

NM_001037339

UniProt #

Q07343

Tag(s)

N-terminal GST-tag, C-terminal His-tag

References

1. Arp, J., et al., Mol Cell Biol. 2003 Nov; 23(22):8042-57.
2. Wang, P., et al., Mol Pharmacol. 1999 Jul; 56(1):170-4.

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4B2 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only