PDE4B1, GST-tag

Catalog #: 60041
Size: 10 µg
$440
Species: Human
Host Species: Sf9 insect cells
MW: 109 kDa
Genbank #: NM_001037341
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human PDE4B1 (GenBank Accession No. NM_001037341), full length with N-terminal GST-tag, MW= 109 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q07343
Synonym(s):
PDE4B1, phosphodiesterase 4B1
Specific Activity:
≥ 8532 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4B1 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Shah,V.O., et al., Am. J. Kidney Dis. 48 (4), 616-628 (2006)
2. Smith,P.G., et al., Blood 105 (1), 308-316 (2005).
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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