PDE4B (Rat), GST-tag Recombinant

Catalog #

60049

$470 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Rat phosphodiesterase 4B (PDE4B) (GenBank Accession No. NM_017031), a.a. 2-722 (end) with N-terminal GST tag, MW=108.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PDE, phosphodiesterase, PDE4-B, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE4B (Rat) inhibition curve using a fluorescence polarization assay (#79571). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Rat

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% Glycerol.

108.3 kDa

Amino Acids

2-722 (end)

Specific Activity

≥ 15,345 pmole/min/µg

Genbank #

NM_017031

UniProt #

P14646

Tag(s)

N-terminal GST-tag

References

1. Girgenti, M.J., et al., PLoS One 2012;7(2):e32404.

2. Zhao, H., et al., Eur. J. Pharmacol. 2008 Aug 20;590(1-3):29-35.

Storage/Stability

>6 months at -80°C

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4B (Rat) at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only