PDE4B (Dog), GST-tag Recombinant

Catalog #

60055

$470 *

Size: 5 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Dog phosphodiesterase 4B (PDE4B) GenBank Accession No. XP_003434692, a.a. 2-737(end), with N-terminal GST-tag, MW 110 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

PDE4B, phosphodiesterase 4B, PDE4-B, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE4B (Dog) inhibition curve using a fluorescence polarization assay (#79573). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Dog

Host Species/Expression System

Sf9 insect cells

Purity

≥50%

Format

Aqueous buffer solution

Formulation

40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT

110 kDa

Amino Acids

2–737(end)

Specific Activity

≥ 3882 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C.

Genbank #

XP_003434692

UniProt #

F1PJ12

Tag(s)

N-terminal GST-tag

References

1. Girgenti, M.J., et al., PLoS One 2012,7(2):e32404.
2. Zhao, H., et al., Eur. J. Pharmacol. 2008 Aug 20,590(1-3):29-35.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of dPDE4B at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PDE

Regulatory Status

Research Use Only