PDE4A1A, GST-tag Recombinant

Catalog #

60040

$485 *

Size: 10 µg

Qty

Bulk/Custom

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This product has been cited 19 times.

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Synonyms

PDE4A1A, phosphodiesterase 4A1A, PDE4-A1A, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE4A1A inhibition curves using a fluorescence polarization assay (#60340). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations19

Species

Human

Construct

PDE4A1A (GST-1-647(end))

Host Species/Expression System

Sf9 insect cells

Purity

≥85%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT

102 kDa

Amino Acids

1-647(end)

Specific Activity

≥9,400 pmol/min/µg

Genbank #

U97584

UniProt #

P27815

Tag(s)

N-terminal GST-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed with 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, 0.1 mg/ml BSA, and PDE4A1A serial diluted from 200 ng – 0 ng. The reaction was incubated at 37°C for 20 min.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only