PDE4A10, GST-tag Recombinant

Catalog #

60038

$470 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human PDE4A10, also known as PDE4A protein isoform 6, transcript variant 3 (GenBank Accession No. NM_001111309), a.a. 2-825(end) with N-terminal GST-tag, MW= 117 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PDE4A protein isoform 6, transcript variant 3, PDE4A10, PDE4-A10, PDE

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol and 3 mM DTT.

117 kDa

Amino Acids

2–825(end)

Specific Activity

≥ 4,400 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Genbank #

NM_001111309

UniProt #

P27815

Tag(s)

N-terminal GST-tag

References

1. McCahill, A., et al. Cell Signal. 2008. Nov; 20(11):2071-83.
2. McPhee, I., et al., Cell Signal. 2001. Dec; 13(12):911-8.

Application Reference(s):

1. Novel multitarget 5-arylidenehydantoins with arylpiperazinealkyl fragment: Pharmacological evaluation and investigation of cytotoxicity and metabolic stability (2019)

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4A10 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only