PDE3A, (669-end), His-GST-tags Recombinant

Catalog #

60032

$455 *

Size: 5 µg

Qty

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Description

Human PDE3A (GenBank Accession No. NM_000921), a.a. 669-1141(end) with N-terminal His-GST-tag, MW = 82 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 10 times.

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Synonyms

PDE3A, phosphodiesterase 3A, PDE3-A, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE3A inhibition curve using a fluorescence polarization assay (#79736). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations10

Species

Human

Construct

PDE3A (His-GST-669-1141(end))

Host Species/Expression System

Sf9

Purity

≥82%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, variable glutathione, 20% glycerol and 3 mM DTT.

82 kDa

Amino Acids

669–1141(end)

Specific Activity

≥2000 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.

Genbank #

NM_000921

UniProt #

Q14432

Tag(s)

N-terminal His-GST-tags

References

1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006)
2. Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE3A at 37°C for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only