PDE2A, GST-Th-Tag Recombinant

Catalog #

60020

$455 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Human PDE2A1 (GenBank Accession No. NM_002599), full length, with N-terminal GST-tag and N-terminal thrombin cleavage site, MW=132 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 9 times.

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Synonyms

PDE2A, phosphodiesterase 2A, PDE2-A, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE2A inhibition curve using a fluorescence polarization assay (#60321). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations9

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥33%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

132 kDa

Amino Acids

full length

Specific Activity

≥ 3442 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Genbank #

NM_002599

UniProt #

O00408

Tag(s)

N-terminal GST-tag

References

1. Seybold, J., et al., Blood 105 (9), 3569- 3576 (2005).
2. Martinez, S.E., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20), 13260-13265 (2002).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE2A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only

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Datasheets/Protocols

60020_Lot130227-G.pdf
60020_Lot130410-GC.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

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PDE2A, GST-Th-Tag Recombinant

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