PDE2A, FLAG-tag Recombinant

Catalog #

60021

$455 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Human PDE2A1 (GenBank Accession No. NM_002599), a.a. 2-941, with N-terminal FLAG-tag, MW=106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PDE2A, phosphodiesterase 2A, PDE2-A, PDE

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥88%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol, and 3 mM DTT.

106 kDa

Amino Acids

2–941

Specific Activity

≥ 7577 pmol/min/μg

Genbank #

NM_002599

UniProt #

O00408

Tag(s)

N-terminal FLAG-tag

References

1. Seybold, J., et al., Blood 105 (9), 3569- 3576 (2005).
2. Martinez, S.E., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20), 13260-13265 (2002).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 μM cAMP, and PDE2A1 at 37ºC for 20 min. Quantified by 5-nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only

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Datasheets/Protocols

60021_Lot102010LN.pdf 60021_Lot100823.pdf

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PDE2A, FLAG-tag Recombinant

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